anti stx18 mouse antibody (Santa Cruz Biotechnology)
Structured Review

Anti Stx18 Mouse Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+stx18/Syntaxin+18+Antibody/pmc12884507-10-0-4
Average 93 stars, based on 15 article reviews
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1) Product Images from "Legionella employs the multimodal ubiquitination of Sec22b to modulate SNARE pairing"
Article Title: Legionella employs the multimodal ubiquitination of Sec22b to modulate SNARE pairing
Journal: iScience
doi: 10.1016/j.isci.2025.114341
Figure Legend Snippet: Ubiquitination of Sec22b promotes non-canonical SNARE pairings with Stx3 and Stx4 (A) HEK293T-FcγRII cells stably expressing FLAG-Sec22b were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h. FLAG-Sec22b derivatives were immunoprecipitated from the cell lysates at the indicated time points using anti-FLAG beads. The cell lysates (input) and the immunoprecipitated proteins (IP: FLAG) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. The intensity values of Stx3 protein bands were quantified using the ChemiDoc System with Image Lab software (Bio-Rad), normalized to the value of the no-infection condition (no bacteria), and displayed below the immunoblotting data. (B) HEK293T-FcγRII cells stably expressing FLAG-Sec22b S137A were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h, and analyzed as described in (A). The intensity values of Stx3 protein bands were quantified as described in (A) and displayed below the immunoblotting data. (C) HEK293T-FcγRII cells stably expressing FLAG-Sec22b or FLAG-Sec22b S137A were infected with the wild-type strain Lp01 at an MOI of 80 for 1 h, and analyzed as described in (A). (D) Intensity values of Stx3, Stx4, and Stx18 protein bands from (C) quantified as described in (A), normalized to the respective Syntaxin values bound to Sec22b WT. Values represent the mean ± SEM from three independent experiments. ns, not significant. ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
Techniques Used: Ubiquitin Proteomics, Stable Transfection, Expressing, Infection, Immunoprecipitation, SDS Page, Western Blot, Software, Bacteria
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other:Article Title: Endoplasmic reticulum–to–Golgi trafficking of procollagen III via conventional vesicular and tubular carriers Article Snippet: Other validated antibodies used were rabbit anti-collagen type III (N-terminal; 22734-1-AP, Proteintech, Rosemont, IL, USA), mouse anti-GFP (11814460001, Roche Applied Science, Mannheim, Germany), Article Title: Endoplasmic reticulum–to–Golgi trafficking of procollagen III via conventional vesicular and tubular carriers Article Snippet: Other validated antibodies used were rabbit anti-collagen type III (N-terminal; 22734-1-AP, Proteintech, Rosemont, IL, USA), mouse anti-GFP (11814460001, Roche Applied Science, Mannheim, Germany), Article Title: Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus Article Snippet: The primary antibodies used were rabbit anti-SNAP29 1:500 , chicken anti-GFP 1:1,000 (Abcam), mouse anti-Vinculin (1:10,000), Article Title: Visualization of ER-to-Golgi trafficking of procollagen X Article Snippet: Other antibodies were purchased from the following suppliers: Article Title: Visualization of ER-to-Golgi trafficking of procollagen X. Article Snippet: Other antibodies were purchased from the following suppliers: Western Blot:Article Title: ATG14 targets lipid droplets and acts as an autophagic receptor for syntaxin18-regulated lipid droplet turnover. Article Snippet: Rabbit anti-GFP (ab6556) was obtained from Abcam. .. Mouse anti-STX18 (sc-293067, WB 1:1000) and Article Title: ATG14 targets lipid droplets and acts as an autophagic receptor for syntaxin18-regulated lipid droplet turnover Article Snippet: Rabbit anti-GFP (ab6556) was obtained from Abcam. .. Mouse anti-STX18 (sc-293067, WB 1:1000) and |