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anti stx18 mouse antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti stx18 mouse antibody
    Ubiquitination of Sec22b promotes non-canonical SNARE pairings with Stx3 and Stx4 (A) HEK293T-FcγRII cells stably expressing FLAG-Sec22b were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h. FLAG-Sec22b derivatives were immunoprecipitated from the cell lysates at the indicated time points using anti-FLAG beads. The cell lysates (input) and the immunoprecipitated proteins (IP: FLAG) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. The intensity values of Stx3 protein bands were quantified using the ChemiDoc System with Image Lab software (Bio-Rad), normalized to the value of the no-infection condition (no bacteria), and displayed below the immunoblotting data. (B) HEK293T-FcγRII cells stably expressing FLAG-Sec22b S137A were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h, and analyzed as described in (A). The intensity values of Stx3 protein bands were quantified as described in (A) and displayed below the immunoblotting data. (C) HEK293T-FcγRII cells stably expressing FLAG-Sec22b or FLAG-Sec22b S137A were infected with the wild-type strain Lp01 at an MOI of 80 for 1 h, and analyzed as described in (A). (D) Intensity values of Stx3, Stx4, and <t>Stx18</t> protein bands from (C) quantified as described in (A), normalized to the respective <t>Syntaxin</t> values bound to Sec22b WT. Values represent the mean ± SEM from three independent experiments. ns, not significant. ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
    Anti Stx18 Mouse Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+stx18/Syntaxin+18+Antibody/pmc12884507-10-0-4
    Average 93 stars, based on 15 article reviews
    anti stx18 mouse antibody - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Legionella employs the multimodal ubiquitination of Sec22b to modulate SNARE pairing"

    Article Title: Legionella employs the multimodal ubiquitination of Sec22b to modulate SNARE pairing

    Journal: iScience

    doi: 10.1016/j.isci.2025.114341

    Ubiquitination of Sec22b promotes non-canonical SNARE pairings with Stx3 and Stx4 (A) HEK293T-FcγRII cells stably expressing FLAG-Sec22b were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h. FLAG-Sec22b derivatives were immunoprecipitated from the cell lysates at the indicated time points using anti-FLAG beads. The cell lysates (input) and the immunoprecipitated proteins (IP: FLAG) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. The intensity values of Stx3 protein bands were quantified using the ChemiDoc System with Image Lab software (Bio-Rad), normalized to the value of the no-infection condition (no bacteria), and displayed below the immunoblotting data. (B) HEK293T-FcγRII cells stably expressing FLAG-Sec22b S137A were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h, and analyzed as described in (A). The intensity values of Stx3 protein bands were quantified as described in (A) and displayed below the immunoblotting data. (C) HEK293T-FcγRII cells stably expressing FLAG-Sec22b or FLAG-Sec22b S137A were infected with the wild-type strain Lp01 at an MOI of 80 for 1 h, and analyzed as described in (A). (D) Intensity values of Stx3, Stx4, and Stx18 protein bands from (C) quantified as described in (A), normalized to the respective Syntaxin values bound to Sec22b WT. Values represent the mean ± SEM from three independent experiments. ns, not significant. ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
    Figure Legend Snippet: Ubiquitination of Sec22b promotes non-canonical SNARE pairings with Stx3 and Stx4 (A) HEK293T-FcγRII cells stably expressing FLAG-Sec22b were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h. FLAG-Sec22b derivatives were immunoprecipitated from the cell lysates at the indicated time points using anti-FLAG beads. The cell lysates (input) and the immunoprecipitated proteins (IP: FLAG) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. The intensity values of Stx3 protein bands were quantified using the ChemiDoc System with Image Lab software (Bio-Rad), normalized to the value of the no-infection condition (no bacteria), and displayed below the immunoblotting data. (B) HEK293T-FcγRII cells stably expressing FLAG-Sec22b S137A were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h, and analyzed as described in (A). The intensity values of Stx3 protein bands were quantified as described in (A) and displayed below the immunoblotting data. (C) HEK293T-FcγRII cells stably expressing FLAG-Sec22b or FLAG-Sec22b S137A were infected with the wild-type strain Lp01 at an MOI of 80 for 1 h, and analyzed as described in (A). (D) Intensity values of Stx3, Stx4, and Stx18 protein bands from (C) quantified as described in (A), normalized to the respective Syntaxin values bound to Sec22b WT. Values represent the mean ± SEM from three independent experiments. ns, not significant. ∗ p < 0.05, ∗∗∗∗ p < 0.0001.

    Techniques Used: Ubiquitin Proteomics, Stable Transfection, Expressing, Infection, Immunoprecipitation, SDS Page, Western Blot, Software, Bacteria

    Related Articles

    other:

    Article Title: Endoplasmic reticulum–to–Golgi trafficking of procollagen III via conventional vesicular and tubular carriers
    Article Snippet: Other validated antibodies used were rabbit anti-collagen type III (N-terminal; 22734-1-AP, Proteintech, Rosemont, IL, USA), mouse anti-GFP (11814460001, Roche Applied Science, Mannheim, Germany), mouse anti-MIA3/ TANGO1 (sc-393916, Santa Cruz Biotech- nology, Dallas, TX, USA), mouse anti-CUL3 (sc-166110, Santa Cruz Biotechnology), mouse anti-KLHL12 (sc-514874, Santa Cruz Biotechnology), rabbit anti-SLY1/SCFD1 (CSB-PA840987ESR2HU, CUSABIO Technology, Wuhan, China), mouse anti-STX18 (sc293067, Santa Cruz Biotechnology), mouse anti-TFG (66919-1-Ig, Proteintech), mouse anti-ZW10 (sc-81430, Santa Cruz Biotechnology), rabbit anti-TRAPPC3/BET3 (CSB-PA000993, CUSABIO Technology), mouse anti-actin (MAB1501, Millipore, Billerica, MA, USA), rabbit anti-calnexin (SPA-860, Enzo Life Sciences, Farmingdale, NY, USA), mouse anti-HSP47 (SPA-470, Enzo Life Sciences), rabbit antiSEC23 (PA1-069, Affinity BioReagents, Golden, CO, USA), mouse 10 | Y. Hirata et al. Molecular Biology of the Cell anti-GM-130 (610823, BD Biosciences, Franklin Lakes, NJ, USA), horseradish peroxidase-conjugated anti-rabbit IgG (BTI-572, BTI; Thermo Fisher Scientific, Rockford, IL, USA), horseradish peroxidaseconjugated anti-mouse IgG (61-6520, Zymed Laboratories/Thermo Fisher Scientific), Alexa Fluor 594-conjugated goat antirabbit IgG (ab150084, Abcam, Cambridge, UK), and Alexa Fluor 488-conjugated goat anti-mouse IgG (ab150117, Abcam).

    Article Title: Endoplasmic reticulum–to–Golgi trafficking of procollagen III via conventional vesicular and tubular carriers
    Article Snippet: Other validated antibodies used were rabbit anti-collagen type III (N-terminal; 22734-1-AP, Proteintech, Rosemont, IL, USA), mouse anti-GFP (11814460001, Roche Applied Science, Mannheim, Germany), mouse anti-MIA3/TANGO1 (sc-393916, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-CUL3 (sc-166110, Santa Cruz Biotechnology), mouse anti-KLHL12 (sc-514874, Santa Cruz Biotechnology), rabbit anti-SLY1/SCFD1 (CSB-PA840987ESR2HU, CUSABIO Technology, Wuhan, China), mouse anti-STX18 (sc-293067, Santa Cruz Biotechnology), mouse anti-TFG (66919-1-Ig, Proteintech), mouse anti-ZW10 (sc-81430, Santa Cruz Biotechnology), rabbit anti-TRAPPC3/BET3 (CSB-PA000993, CUSABIO Technology), mouse anti-actin (MAB1501, Millipore, Billerica, MA, USA), rabbit anti-calnexin (SPA-860, Enzo Life Sciences, Farmingdale, NY, USA), mouse anti-HSP47 (SPA-470, Enzo Life Sciences), rabbit anti-SEC23 (PA1-069, Affinity BioReagents, Golden, CO, USA), mouse anti-GM-130 (610823, BD Biosciences, Franklin Lakes, NJ, USA), horseradish peroxidase-conjugated anti-rabbit IgG (BTI-572, BTI; Thermo Fisher Scientific, Rockford, IL, USA), horseradish peroxidase-conjugated anti-mouse IgG (61-6520, Zymed Laboratories/Thermo Fisher Scientific), Alexa Fluor 594-conjugated goat anti-rabbit IgG (ab150084, Abcam, Cambridge, UK), and Alexa Fluor 488-conjugated goat anti-mouse IgG (ab150117, Abcam).

    Article Title: Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus
    Article Snippet: The primary antibodies used were rabbit anti-SNAP29 1:500 , chicken anti-GFP 1:1,000 (Abcam), mouse anti-Vinculin (1:10,000), mouse anti-STX18 1:500 (Santa Cruz), and mouse anti-αtubulin 1:8,000 (Cell Signaling #3873), rabbit anti-STX5 1:1,000, rabbit anti-SEC22B 1:1,000, rabbit anti-USE1 1:500, rabbit anti-BNIP 1:500, and rabbit anti-STX18 1:500 (all from SYSY), rabbit anti-Snap29 1:1,000 , and mouse anti-HA 1:500 (Covance).

    Article Title: Visualization of ER-to-Golgi trafficking of procollagen X
    Article Snippet: Other antibodies were purchased from the following suppliers: mouse anti-MIA3/TANGO1 (Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-CUL3 (Santa Cruz Biotechnology), rabbit anti-SLY1/SCFD1 (CUSABIO Technology, Wuhan, China), mouse anti-STX18 (Santa Cruz Biotechnology), mouse anti-ZW10 (Santa Cruz Biotechnology), rabbit anti-TRAPPC3/BET3 (CUSABIO Technology), mouse anti-actin (Millipore, Billerica, MA, USA), rabbit anti-calnexin (Enzo Life Sciences, Farmingdale, NY, USA), rabbit anti-SEC23 (Affinity BioReagents, Golden, CO, USA), rabbit anti-calreticulin (CRT, Affinity BioReagents), horseradish peroxidase-conjugated anti-rabbit IgG (BTI; Thermo Fisher Scientific, Rockford, IL, USA), horseradish peroxidase-conjugated anti-mouse IgG (Zymed Laboratories/Thermo Fisher Scientific), Alexa Fluor 594-conjugated goat anti-rabbit IgG (Thermo Fisher Scientific), and Alexa Fluor 488-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).

    Article Title: Visualization of ER-to-Golgi trafficking of procollagen X.
    Article Snippet: Other antibodies were purchased from the following suppliers: mouse anti-MIA3/TANGO1 (Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-CUL3 (Santa Cruz Biotechnology), rabbit anti-SLY1/SCFD1 (CUSABIO Technology, Wuhan, China), mouse anti-STX18 (Santa Cruz Biotechnology), mouse anti-ZW10 (Santa Cruz Biotechnology), rabbit anti-TRAPPC3/BET3 (CUSABIO Technology), mouse anti-actin (Millipore, Billerica, MA, USA), rabbit anti-calnexin (Enzo Life Sciences, Farmingdale, NY, USA), rabbit anti-SEC23 (Affinity BioReagents, Golden, CO, USA), rabbit anti-calreticulin (CRT, Affinity BioReagents), horseradish peroxidase-conjugated anti-rabbit IgG (BTI; Thermo Fisher Scientific, Rockford, IL, 9 USA), horseradish peroxidase-conjugated anti-mouse IgG (Zymed Laboratories/Thermo Fisher Scientific), Alexa Fluor 594-conjugated goat anti-rabbit IgG (Thermo Fisher Scientific), and Alexa Fluor 488-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).

    Western Blot:

    Article Title: ATG14 targets lipid droplets and acts as an autophagic receptor for syntaxin18-regulated lipid droplet turnover.
    Article Snippet: Rabbit anti-GFP (ab6556) was obtained from Abcam. .. Mouse anti-STX18 (sc-293067, WB 1:1000) and mouse anti-GAPDH (sc-365062, WB 1:5000) were obtained from Santa Cruz Biotechnology. .. Rabbit antiVps34 (Z-R015, WB 1:1000) was obtained from Echelon Bioscience.

    Article Title: ATG14 targets lipid droplets and acts as an autophagic receptor for syntaxin18-regulated lipid droplet turnover
    Article Snippet: Rabbit anti-GFP (ab6556) was obtained from Abcam. .. Mouse anti-STX18 (sc-293067, WB 1:1000) and mouse anti-GAPDH (sc-365062, WB 1:5000) were obtained from Santa Cruz Biotechnology. .. Rabbit anti-Vps34 (Z-R015, WB 1:1000) was obtained from Echelon Bioscience.



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    Ubiquitination of Sec22b promotes non-canonical SNARE pairings with Stx3 and Stx4 (A) HEK293T-FcγRII cells stably expressing FLAG-Sec22b were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h. FLAG-Sec22b derivatives were immunoprecipitated from the cell lysates at the indicated time points using anti-FLAG beads. The cell lysates (input) and the immunoprecipitated proteins (IP: FLAG) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. The intensity values of Stx3 protein bands were quantified using the ChemiDoc System with Image Lab software (Bio-Rad), normalized to the value of the no-infection condition (no bacteria), and displayed below the immunoblotting data. (B) HEK293T-FcγRII cells stably expressing FLAG-Sec22b S137A were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h, and analyzed as described in (A). The intensity values of Stx3 protein bands were quantified as described in (A) and displayed below the immunoblotting data. (C) HEK293T-FcγRII cells stably expressing FLAG-Sec22b or FLAG-Sec22b S137A were infected with the wild-type strain Lp01 at an MOI of 80 for 1 h, and analyzed as described in (A). (D) Intensity values of Stx3, Stx4, and <t>Stx18</t> protein bands from (C) quantified as described in (A), normalized to the respective <t>Syntaxin</t> values bound to Sec22b WT. Values represent the mean ± SEM from three independent experiments. ns, not significant. ∗ p < 0.05, ∗∗∗∗ p < 0.0001.
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    Image Search Results


    Ubiquitination of Sec22b promotes non-canonical SNARE pairings with Stx3 and Stx4 (A) HEK293T-FcγRII cells stably expressing FLAG-Sec22b were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h. FLAG-Sec22b derivatives were immunoprecipitated from the cell lysates at the indicated time points using anti-FLAG beads. The cell lysates (input) and the immunoprecipitated proteins (IP: FLAG) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. The intensity values of Stx3 protein bands were quantified using the ChemiDoc System with Image Lab software (Bio-Rad), normalized to the value of the no-infection condition (no bacteria), and displayed below the immunoblotting data. (B) HEK293T-FcγRII cells stably expressing FLAG-Sec22b S137A were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h, and analyzed as described in (A). The intensity values of Stx3 protein bands were quantified as described in (A) and displayed below the immunoblotting data. (C) HEK293T-FcγRII cells stably expressing FLAG-Sec22b or FLAG-Sec22b S137A were infected with the wild-type strain Lp01 at an MOI of 80 for 1 h, and analyzed as described in (A). (D) Intensity values of Stx3, Stx4, and Stx18 protein bands from (C) quantified as described in (A), normalized to the respective Syntaxin values bound to Sec22b WT. Values represent the mean ± SEM from three independent experiments. ns, not significant. ∗ p < 0.05, ∗∗∗∗ p < 0.0001.

    Journal: iScience

    Article Title: Legionella employs the multimodal ubiquitination of Sec22b to modulate SNARE pairing

    doi: 10.1016/j.isci.2025.114341

    Figure Lengend Snippet: Ubiquitination of Sec22b promotes non-canonical SNARE pairings with Stx3 and Stx4 (A) HEK293T-FcγRII cells stably expressing FLAG-Sec22b were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h. FLAG-Sec22b derivatives were immunoprecipitated from the cell lysates at the indicated time points using anti-FLAG beads. The cell lysates (input) and the immunoprecipitated proteins (IP: FLAG) were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. The intensity values of Stx3 protein bands were quantified using the ChemiDoc System with Image Lab software (Bio-Rad), normalized to the value of the no-infection condition (no bacteria), and displayed below the immunoblotting data. (B) HEK293T-FcγRII cells stably expressing FLAG-Sec22b S137A were infected with the indicated L. pneumophila strains at an MOI of 80 for 1 h, and analyzed as described in (A). The intensity values of Stx3 protein bands were quantified as described in (A) and displayed below the immunoblotting data. (C) HEK293T-FcγRII cells stably expressing FLAG-Sec22b or FLAG-Sec22b S137A were infected with the wild-type strain Lp01 at an MOI of 80 for 1 h, and analyzed as described in (A). (D) Intensity values of Stx3, Stx4, and Stx18 protein bands from (C) quantified as described in (A), normalized to the respective Syntaxin values bound to Sec22b WT. Values represent the mean ± SEM from three independent experiments. ns, not significant. ∗ p < 0.05, ∗∗∗∗ p < 0.0001.

    Article Snippet: Anti-Stx18 mouse antibody , Santa Cruz Biotechnology , Cat# sc-293067; RRID:AB_10647235.

    Techniques: Ubiquitin Proteomics, Stable Transfection, Expressing, Infection, Immunoprecipitation, SDS Page, Western Blot, Software, Bacteria